Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

YU Zhi-Wu; FENG Ying; WANG Jun; DONG Bao-Zhong

Chinese Physics C> 2001, Vol. 25> Issue(S1) : 75-80

Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

Department of Chemistry,Tsinghua University,Beijing 100084,China2 Institute of High Energy Physics,CAS,Beijing 100039,China3 Division of Life Sciences,King's College London,150 Stanford St.,LondonSEI 8WA,UK

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Abstract：
Synchrotron X-ray diffraction and differential scanning calorimetry were used to study the effect of urea solutions on the phase behavior of Dioleoylglycero-phosphatidvlethanolamine (DOPE).It was found that urea could stabilize lamellar liquid-crystalline phase at the expense of lamellar-gel phase and non-bilayer inverted hexagonal phase.The repeat distance of DOPE multilamellar structure increases with the increasing of urea concentration.The molecular mechanisms are discussed in terms of the possible hydrogen bonds between urea and the phospholipid head groups and the Hofmeister effect of urea,where urea is taken as a waterstructure breaker.In addition,X-ray diffraction pattern of a wet rat tail collagen fiber was collected and was used as calibration standard at small-angle diffraction stations.

References

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YU Zhi-Wu, FENG Ying, WANG Jun and DONG Bao-Zhong. Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction[J]. Chinese Physics C, 2001, 25(S1): 75-80.

YU Zhi-Wu, FENG Ying, WANG Jun and DONG Bao-Zhong. Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction[J]. Chinese Physics C, 2001, 25(S1): 75-80. shu

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Received: 1900-01-01
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通讯作者: 陈斌, [email protected]

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沈阳化工大学材料科学与工程学院沈阳 110142

Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

Corresponding author: YU Zhi-Wu,

Department of Chemistry,Tsinghua University,Beijing 100084,China2 Institute of High Energy Physics,CAS,Beijing 100039,China3 Division of Life Sciences,King's College London,150 Stanford St.,LondonSEI 8WA,UK

Received Date: 1900-01-01

Accepted Date: 1900-01-01

Available Online: 2001-06-22

Abstract: Synchrotron X-ray diffraction and differential scanning calorimetry were used to study the effect of urea solutions on the phase behavior of Dioleoylglycero-phosphatidvlethanolamine (DOPE).It was found that urea could stabilize lamellar liquid-crystalline phase at the expense of lamellar-gel phase and non-bilayer inverted hexagonal phase.The repeat distance of DOPE multilamellar structure increases with the increasing of urea concentration.The molecular mechanisms are discussed in terms of the possible hydrogen bonds between urea and the phospholipid head groups and the Hofmeister effect of urea,where urea is taken as a waterstructure breaker.In addition,X-ray diffraction pattern of a wet rat tail collagen fiber was collected and was used as calibration standard at small-angle diffraction stations.

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Study of the Effect of Urea on the Phase Transition of Phospholipid by Synchrotron X-Ray Diffraction

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